Specimen Nr. 01E

Specimen:

Kidney (Rat)

Staining:

Hopa

Magnification:

160x

Important structures :

1.	Vascular pole of a glomerulus
2.	Urinary (tubular) pole of a glomerulus
3.	Bowman capsule with basement membrane (parietal layer)
4.	Bowman capsular space
5.	Glomerulus - capillaries
6.	Mesangium
7.	Macula densa (part of juxtaglomerular complex)
8.	Extraglomular mesangial cells (Lacis cells) with basement membrane
9.	Proximal tubule (proximal convoluted tubule)
10.	Distal convoluted tubule

Das parietale Blatt der Bowman?schen Kapsel besteht aus einem flachen einschichtigen Epithel, von dem man nur die Zellkerne erkennen kann, sowie aus einer Basalmembran. Das innere (viszerale) Blatt der Bowman?schen Kapsel wird durch die Zellkerne der Podozyten repräsentiert. Diese sind jedoch kaum von den Zellkernen der Endothelzellen und der Mesangiumzellen zu unterscheiden. Gelb gefärbt sind die Erythrozyten im Lumen der Kapillaren, blau gefärbt sind Basalmembran und mesangiale Matrix. Am Gefäßpol sind Vas afferens und efferens, nicht angeschnitten.

Legende:

	Vascular pole of a glomerulus
	Urinary (tubular) pole of a glomerulus
	Bowman capsule with basement membrane (parietal layer)
	Bowman capsular space
	Glomerulus - capillaries
	Mesangium
	Macula densa (part of juxtaglomerular complex)
	Extraglomular mesangial cells (Lacis cells) with basement membrane
	Proximal tubule (proximal convoluted tubule)
	Distal convoluted tubule

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der Raum zwischen viszeralem und parietalem Blatt der Bowman?schen Kapsel enthält den Primärharn. Von den drei Bestandteilen des juxtaglomerulären Apparat sind Macula densa und extraglomeruläres Mesangium zu erkennen, nicht aber die glatten Muskelzellen mit endokriner Funktion (Renin-Sekretion), die die Tunica media der Arteriola afferens und efferens bilden. Das Renin-Angiotensin-System ist von entscheidender Bedeutung für die Regulierung des Blutdrucks. Die Macula densa dient möglicherweise als Element einer Rückkopplungsschleife, über die die Na⁺-Konzentration im distalen Tubulus die Durchblutung des Glomerulus reguliert. Die extraglomerulären Mesangiumzellen wurden lange Zeit als Ort der Erythropoetinbildung angesehen. Die Epithelzellen des distalen Tubulus sind Zielzellen für Aldosteron (u.a. Rückresorption von Na⁺-Ionen).

Vascular pole of a glomerulus

Urinary (tubular) pole of a glomerulus

Bowman capsule with basement membrane (parietal layer)

Bowman capsular space

Glomerulus - capillaries

Mesangium

Macula densa (part of juxtaglomerular complex)

Extraglomular mesangial cells (Lacis cells) with basement membrane

Proximal tubule (proximal convoluted tubule)

Distal convoluted tubule

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Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
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