Specimen Nr. 01C

Specimen:

Kidney (Rat)

Staining:

Hopa

Magnification:

32x

Important structures :

1.	Glomerulus
2.	Proximal tubule (proximal convoluted tubule)
3.	Distal convoluted tubule
4.	Connective tissue
5.	Capillary (peritubular)
6.	White adipose tissue
7.	Brown adipose tissue

Die in der Rinde liegenden Kanälchenabschnitte zeigen wegen ihres gewundenen Verlaufes sehr vielfältig geformte Anschnitte. Proximale und distale Tubulusabschnitte sind leicht zu unterscheiden, weil die proximalen Tubulusabschnitte ein höheres Epithel mit Bürstensaum und damit ein deutlich engeres Lumen aufweisen.

Legende:

	Glomerulus
	Proximal tubule (proximal convoluted tubule)
	Distal convoluted tubule
	Connective tissue
	Capillary (peritubular)
	White adipose tissue
	Brown adipose tissue

Parts of the nephron in the renal cortex[sl]

1.	Arcuate artery
2.	Interlobular artery (cortical radiate artery)
3.	Afferent arteriole
4.	Distal convoluted tubule
5.	Connecting tubule
6.	Proximal tubule (proximal convoluted tubule)
7.	Efferent arteriole
8.	Straight medullary artery

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

In der Rinde kommen fast nur Glomeruli und gewundene Abschnitte der proximalen und distalen Tubuli vor. Sammelrohre sind selten. Verbindungsstücke sind im allgemeinen schwer zu finden. Sie können nur erkannt werden, wenn ihre Mündung in ein Sammelrohr zufällig angeschnitten ist.

Magnification:

32x

Magnification:

64x

Magnification:

160x

Proximal tubule (proximal convoluted tubule)

Distal convoluted tubule

Capillary (peritubular)

White adipose tissue

Brown adipose tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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