Information
Drawing
Staining
Knowledge
marked
unmarked

Specimen Nr. 01F

Specimen:

Kidney (Rat)

Staining:

Hopa

Magnification:

160x

Important structures :

1.Proximal tubule
2.Distal tubule
3.Capillary (peritubular)
Proximale und distale Tubulusabschnitte sind leicht zu unterscheiden, weil die proximalen Tubulusabschnitte ein höheres Epithel mit Bürstensaum und damit ein deutlich engeres Lumen aufweisen.

Legende:

Proximal tubule
Distal tubule
Capillary (peritubular)

Sections of renal cortex showing proximal and distal convoluted tubules[bu]

1. Capillary
2. Proximal tubule
3. Distal tubule
4. Intermediate tubule
5. Lysosomes

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der distale Tubulus kehrt im Anschluss an die Henlesche Schleife wieder in die unmittelbare Nachbarschaft desjenigen Glomerulus zurück, von dem das Nephron ausgegangen ist. Er bildet dort am Gefäßpol die Macula densa. Diese ist daran zu erkennen, daß die Zellkerne des Epithels ungewöhnlich dicht stehen. Die Macula densa dient möglicherweise als Element einer Rückkopplungsschleife, über die die Na+-Konzentration im distalen Tubulus die Durchblutung des Glomerulus reguliert.
Home
Tutor
Help
Exit
Boxes

Magnification:

64x

Magnification:

160x

Magnification:

160x

Magnification:

10x

Magnification:

50x

Magnifications
Proximal tubule
Proximal tubule
Distal tubule
Distal tubule
Distal tubule
Capillary (peritubular)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help