Specimen Nr. 01D

Specimen:

Kidney (Rat)

Staining:

Hopa

Magnification:

64x

Important structures :

1.	Glomerulus
2.	Urinary (tubular) pole of a glomerulus
3.	Macula densa (on vascular pole)
4.	Capillaries and mesangium of glomerulus
5.	Proximal tubule (proximal convoluted tubule)
6.	Distal convoluted tubule
7.	Capillaries (peritubular)

Man sieht die drei Nephron-Abschnitte, die für die Rinde typisch sind: Glomeruli, proximale und distale gewundene Tubuli (= Pars convoluta der Tubuli). Die Kapillaren des Glomerulus sowie die peritubulären Kapillaren fallen durch die goldgelbe Färbung der Erythrozyten auf. Blau gefärbt sind Proteoglycan-haltige Strukturen: Basalmembran der Tubuli, Basalmembranen und mesangiale Matrix des Glomerulus.

Legende:

	Glomerulus
	Urinary (tubular) pole of a glomerulus
	Macula densa (on vascular pole)
	Capillaries and mesangium of glomerulus
	Proximal tubule (proximal convoluted tubule)
	Distal convoluted tubule
	Capillaries (peritubular)

Sections of renal cortex showing proximal and distal convoluted tubules[bu]

1.	Capillary
2.	Proximal tubule
3.	Distal tubule
4.	Intermediate tubule
5.	Lysosomes

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der distale Tubulus kehrt im Anschluss an die Henlesche Schleife wieder in die unmittelbare Nachbarschaft desjenigen Glomerulus zurück, von dem das Nephron ausgegangen ist. Er bildet dort am Gefäßpol die Macula densa. Diese ist daran zu erkennen, daß die Zellkerne des Epithels ungewöhnlich dicht stehen.

Magnification:

32x

Magnification:

64x

Magnification:

160x

Magnification:

160x

Urinary (tubular) pole of a glomerulus

Macula densa (on vascular pole)

Capillaries and mesangium of glomerulus

Proximal tubule (proximal convoluted tubule)

Distal convoluted tubule

Capillaries (peritubular)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
Exit: closes down the HistoNet program
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VM: provides virtual microscopy

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