Specimen Nr. 01A

Specimen:

Kidney (Rat)

Staining:

Hopa

Magnification:

4x

Important structures :

1.Fibrous renal capsule
2.Renal cortex
3.Outer renal medulla
4.Internal renal medulla
5.Renal papilla with renal calyx
6.Renal artery (in renal sinus)
7.Ureter
8.Renal vein in renal sinus
9.White adipose tissue
10.Brown adipose tissue
Übersichtsaufnahme einer Rattenniere, auf der die Nierenrinde (Cortex renalis) und das Nierenmark (Medulla renalis) des Nierenparenchyms zu erkennen sind. Im rechten oberen Quadranten sieht man die Einbuchtung des Sinus renalis. Er liegt ausserhalb des Nierenparenchyms und wird von Fettgewebe ausgefüllt. Hier liegen die A. und V. renalis (bzw. deren Äste), der Harnleiter (Ureter) und ein Nierenkelch (Calix renalis). Die Nierenpapille (Papilla renalis) ist vom Nierenkelch umgeben. Zwischen Nierenpapille und Nierenkelch liegt ein Spalt, der den Urin aufnimmt.

Legende:

Fibrous renal capsule
Renal cortex
Outer renal medulla
Internal renal medulla
Renal papilla with renal calyx
Renal artery (in renal sinus)
Ureter
Renal vein in renal sinus
White adipose tissue
Brown adipose tissue

Schematic view of kidney[li]

1. Renal cortex
2. Renal pyramid
3. Renal sinus
4. Renal calyx
5. Renal pelvis
6. Ureter

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der Sinus renalis enthält außer Fettgewebe die Strukturen des Nierenhilus: Nierenarterie und Nierenvene (bzw. ihre Zweige), Nierenbecken (auf dieser Abbildung nicht zu sehen), Nierenkelche, Ureter. Beachte: die Rattenniere ist im Gegensatz zur menschlichen Niere eine unipapilläre Niere und weist somit auch nur einen Kelch auf.
Home
Tutor
Help
Exit
Boxes

Magnification:

4x

Magnification:

4x

Magnification:

32x

Magnification:

64x

Magnification:

160x

Magnifications
Fibrous renal capsule
Renal cortex
Outer renal medulla
Internal renal medulla
Renal papilla with renal calyx
Renal artery (in renal sinus)
Ureter
Renal vein in renal sinus
Renal vein in renal sinus
White adipose tissue
Brown adipose tissue

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help