Auf dem Bild sind die Lamina epithelialis und die Lamina propria der Tunica mucosa der Trachea angeschnitten. Das mehrreihige hochprismatische Epithel der Tunica mucosa trägt Kinozilien. Eingestreut sind blaß gefärbte Becherzellen. Die Lamina propria enthält Fibrozyten mit länglichen sowie Lymphozyten mit runden Kernen. Letztere liegen zum Teil auch intraepithelial.

Legende:

	Ciliated pseudostratified columnar epithelium
	Lamina propria
	Lymphocytes of lamina propria
	Seromucous tracheal glands
	Loose connective tissue with fibrocytes of lamina propria
	Capillary

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Wandaufbau der Trachea:

Tunica mucosa:
1. Lamina epithelialis mit mehrreihigem, hochprismatischem Epithel mit Kinozilien
2. Lamina propria mit kollagenen und elastischen Fasernetzen, Glandulae tracheales, Lymphozyten, Fibrozyten und Kapillaren
Tunica fibromusculocartilaginea: hier befinden sich die hufeisenförmig angeordneten Spangen aus hyalinem Knorpel. Sie sind untereinander durch kollagene sowie elastische Fasergeflechte verbunden. Die freien Enden der Hufeisen verbindet ein Muskel, der Musculus trachealis.
Tunica adventitia: sie besteht aus lockerem Bindegewebe, um die Verschiebungen der Trachea gegen die Umgebung während des Schluckaktes zu ermöglichen.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 01C

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Hematoxylin and Eosin (H&E)

Dyes:

HistoNet2000 - Help