Der Ausschnitt zeigt die mehr oberflächennah gelegenen Zellagen des unverhornten mehrschichtigen Plattenepithels der Speiseröhrenschleimhaut. Teils erscheinen die Zellen polyedrisch mit großen runden Zellkernen, teils platt. Während viele an das Lumen grenzende Zellen Zellkerne aufweisen, sind bei anderen nur noch Kernfragmente zu sehen. Alle oberflächennahen Zellen sind weniger intensiv gefärbt als die unteren, basalen, hier nicht abgebildeten Zellagen.

Legende:

	Squamous cell with nucleus bordering on the oesophageal lumen
	Squamous cell with nuclear fragment bordering on the oesophageal lumen
	Polyhedric cells

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Der Übergang Oesophagus ? Magen ist eine wichtige Stelle für pathologische Veränderungen. In diesem Bereich findet ein Übergang des Plattenepithels in ein Zylinderepithel, ähnlich dem des Anfangsteils des Magens, statt. Die Erkrankungen (z.B. Ulcera, Strikturen und Krebs) entstehen hier dadurch, daß das Plattenepithel des unteren Oesophagus der Säure und den Verdauungsenzymen ausgesetzt ist.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 05D

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Hematoxylin and Eosin (H&E)

Dyes:

HistoNet2000 - Help