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Specimen Nr. 02D

Specimen:

Spinal ganglion (Rabbit)

Staining:

Azan

Magnification:

350x

Important structures :

1.Cytoplasm of ganglion cell with Nissl bodies
2.Nucleus with distinct nucleolus of a ganglion cell
3.Nucleus of satellite cell
4.Collagen fibres of endoneurium
Die Nissl-Substanz besteht aus feinen Punkten, von denen jeder im elektronenmikroskopischen Bild einem kleinen Stapel von granuliertem (rauhem) endoplasmatischem Retikulum oder einer Ansammlung von Polyribosomen entspräche. (Überlege, warum ein dicker deutlich gefärbter Nucleolus und der Reichtum an Ribosomen zusammenpaßt!) Auffallend ist die unterschiedliche Größe der Zellkerne: Die Kerne der Gliazellen haben etwa den Durchmesser des Nucleolus der Nervenzelle.

Legende:

Cytoplasm of ganglion cell with Nissl bodies
Nucleus with distinct nucleolus of a ganglion cell
Nucleus of satellite cell
Collagen fibres of endoneurium

Ganglion[bu]

1. Ganglion cell (neuron)
2. Satellite cells
3. Nucleus of Schwann cell
4. Nerve fibre
5. Lipofuscin (age pigment)
6. Capillary
7. Endoneurium
8. Axon hillock of a ganglion cell
9. Slice of ganglion cell (neuron)

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic
blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G		 This dye is used for counterstaining.
Die Nervenzellen des Spinalganglions verwenden Glutamat als Neurotransmitter, häufig zusammen mit Neuropeptiden. (Beachte: innerhalb des Spinalganglions gibt es keine Synapsen!) Die Mantelzellen gehören zur peripheren Glia. Sie sind wichtig für die Lebensfähigkeit der Nervenzellen. Mittels parakriner Sekretion sezernieren sie z. B. neurotrophe Faktoren (Wachstumsfaktoren), auf die die Nervenzelle angewiesen ist. Umgekehrt entfernen sie Substanzen aus dem Extrazellulärraum, die der Nervenzelle gefährlich werden könnten, wenn sie toxische Konzentrationen erreichen.
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Magnification:

11x

Magnification:

44x

Magnification:

70x

Magnification:

350x

Magnifications
Cytoplasm of ganglion cell with Nissl bodies
Cytoplasm of ganglion cell with Nissl bodies
Nucleus with distinct nucleolus of a ganglion cell
Nucleus of satellite cell
Nucleus of satellite cell
Collagen fibres of endoneurium
Collagen fibres of endoneurium
Collagen fibres of endoneurium
Collagen fibres of endoneurium

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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