Specimen Nr. 02A

Specimen:

Spinal ganglion (Rabbit)

Staining:

Azan

Magnification:

11x

Important structures :

1.Dura mater
2.Ganglion cells
3.Nerve fibres (axons)
Dieser Querschnitt zeigt eines der 32 Spinalganglien, die rechts und links in jedem Zwischenwirbelloch (Foramen intervertebrale) der Wirbelsäule in unmittelbarer Nähe des Rückenmarks liegen. Jedes Spinalganglion wird von der Dura mater, der äußeren Rückenmarkshaut, überzogen. (Diese setzt sich in das Epineurium des peripheren Nerven fort). Es sind zahlreiche große Ganglienzellen, deren Zellkörper hell erscheinen, sowie Bündel längsgeschnittener Nervenfasern (Axone) zu erkennen.

Legende:

Dura mater
Ganglion cells
Nerve fibres (axons)

Ganglion[bu]

1. Ganglion cell (neuron)
2. Satellite cells
3. Nucleus of Schwann cell
4. Nerve fibre
5. Lipofuscin (age pigment)
6. Capillary
7. Endoneurium
8. Axon hillock of a ganglion cell
9. Slice of ganglion cell (neuron)

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic

blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G
This dye is used for counterstaining.
Die Ganglienzellen in dem Spinalganglion gehören teils zum somatischen teils zum vegetativen Nervensystem. Sie vermitteln viszero-afferente oder somato-afferente Informationen, je nachdem, ob ihre Axone in den Eingeweiden oder in der Skelettmuskulatur bzw. in der Haut enden. Bei den Spinalganglienzellen handelt es sich um pseudounipolare Nervenzellen, die nur einen Fortsatz (Axon) aufweisen. Dieser zweigt sich kurz nach Abgang vom Zellkörper (Perikaryon) T-förmig auf.
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Magnification:

11x

Magnification:

44x

Magnification:

70x

Magnification:

350x

Magnifications
Dura mater
Dura mater
Ganglion cells
Ganglion cells
Ganglion cells
Nerve fibres (axons)
Nerve fibres (axons)
Nerve fibres (axons)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
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VM: provides virtual microscopy

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