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Specimen Nr. 02B

Specimen:

Spinal ganglion (Rabbit)

Staining:

Azan

Magnification:

44x

Important structures :

1.Nucleus with nucleolus of a nerve cell
2.Neuron
3.Small slightly shrunken nerve cell
4.Endoneurium
5.Bundle of longitudinally sliced nerve fibres (axons)
6.Nuclei of satellite cells
Nervenzellen erkennt man an ihrem hellen, chromatinarmen Zellkern und, sofern angeschnitten, an dem deutlichen Nucleolus. Bei manchen Zellen ist der Abgang des Axons zu sehen. Die kleinen Nervenzellen sind häufig stärker gefärbt und weisen einen deutlichen, artifiziell bedingten Schrumpfspalt zwischen Zellkörper und umgebenden Gliazellen auf. Die feinen, blauen, faserigen Strukturen sind Kollagenfasern und gehören zum Endoneurium. Weiterhin sind Bündel längs geschnittener Nervenfasern (Axone) zu erkennen. Die längs geschnittenen Nervenfasern zeigen ein rot gefärbtes Axon, das von einem hellen Hof umgeben ist. Der helle Hof ist durch das Herauslösen des Myelins bei der histologischen Präparation entstanden.

Legende:

Nucleus with nucleolus of a nerve cell
Neuron
Small slightly shrunken nerve cell
Endoneurium
Bundle of longitudinally sliced nerve fibres (axons)
Nuclei of satellite cells

Ganglion[bu]

1. Ganglion cell (neuron)
2. Satellite cells
3. Nucleus of Schwann cell
4. Nerve fibre
5. Lipofuscin (age pigment)
6. Capillary
7. Endoneurium
8. Axon hillock of a ganglion cell
9. Slice of ganglion cell (neuron)

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic
blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G		 This dye is used for counterstaining.
Die Größe der neuronalen Zellkörper variiert mit der Dicke der Axone. Man nimmt an, daß von den kleinen Nervenzellen hauptsächlich dünne, wenig myelinisierte Axone ausgehen, z.B. Schmerzfasern (Vergleiche die Lehrbücher der Physiologie bezüglich der Zuordnung von Axondurchmesser und Leitungsqualitäten.) Längere Axone sind anfälliger gegenüber schädigenden Einflüssen als kurze.
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Magnification:

11x

Magnification:

44x

Magnification:

70x

Magnification:

350x

Magnifications
Nucleus with nucleolus of a nerve cell
Neuron
Small slightly shrunken nerve cell
Endoneurium
Bundle of longitudinally sliced nerve fibres (axons)
Bundle of longitudinally sliced nerve fibres (axons)
Nuclei of satellite cells

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
Exit: closes down the HistoNet program
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VM: provides virtual microscopy

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