Specimen Nr. 01B

Specimen:

Pituitary gland (Human being)

Staining:

Azan

Magnification:

32x

Important structures :

1.	Capsule
2.	Group of undifferentiated cells (stem cells)
3.	Group of basophilic cells
4.	Eosinophilic cells
5.	Sinusoid capillaries

Die endokrinen Zellen sind durch retikuläres Bindegewebe zu Gruppen zusammengefaßt. Aufgrund unterschiedlicher Färbeeigenschaften erkennt man verschiedene Zelltypen. Dazwischen liegen zahlreiche weite Kapillaren (Sinusoide).

Legende:

	Capsule
	Group of undifferentiated cells (stem cells)
	Group of basophilic cells
	Eosinophilic cells
	Sinusoid capillaries

Endocrine System

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Die unterschiedliche Anfärbung mit konventionellen Farbstoffen (eosinophil, basophil) ist heutzutage nicht mehr wichtig. Dagegen erlaubt die immunhistochemische Anfärbung mit Antikörpern gegen das jeweilige Hormon die verschiedenen Zelltypen einwandfrei zu identifizieren.

Magnification:

32x

Magnification:

80x

Group of undifferentiated cells (stem cells)

Group of basophilic cells

Sinusoid capillaries

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!