Specimen Nr. 01A

Specimen:

Pituitary gland (Human being)

Staining:

Azan

Magnification:

Important structures :

1.	Infundibulum of pituitary gland
2.	Pars tuberalis
3.	Capsule (dura mater)
4.	Adenohypophysis (Anterior lobe, pars distalis)
5.	Neurohypophysis (pars nervosa)
6.	Pars intermedia with colloid follicles
7.	Colloid droplet

Die epitheliale Struktur der Adenohypophyse und die "faserige" Struktur der aus Nervengewebe bestehenden Neurohypophyse sind leicht zu unterscheiden. Der ebenfalls zur Adenohypophyse zählende Zwischenlappen fällt durch seine Kolloidfollikel auf.

Legende:

	Infundibulum of pituitary gland
	Pars tuberalis
	Capsule (dura mater)
	Adenohypophysis (Anterior lobe, pars distalis)
	Neurohypophysis (pars nervosa)
	Pars intermedia with colloid follicles
	Colloid droplet

Endocrine System

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Adeno- und Neurohypophyse sind entwicklungsgeschichtlich verschiedener Herkunft. Die Adenohypophyse entstammt dem Ektoderm des Rachendaches, die Neurohypophyse ist Teil des ZNS (Hypothalamus).

Magnification:

32x

Magnification:

80x

Infundibulum of pituitary gland

Capsule (dura mater)

Adenohypophysis (Anterior lobe, pars distalis)

Neurohypophysis (pars nervosa)

Pars intermedia with colloid follicles

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!