Specimen Nr. 02B

Specimen:

Kidney (Pig)

Staining:

H&E

Magnification:

81x

Important structures :

1.	Capillaries and mesangium of glomerulus
2.	Urinary (tubular) pole of a glomerulus
3.	Macula densa
4.	Extraglomular mesangial cells (Lacis cells) on vascular pole of a glomerulus
5.	Bowman capsular space
6.	Proximal tubule (proximal convoluted tubule)
7.	Distal convoluted tubule

Man sieht die drei Nephron-Abschnitte, die für die Rinde typisch sind: Glomeruli, proximale und distale gewundene Tubuli (= Pars convoluta der Tubuli).

Legende:

	Capillaries and mesangium of glomerulus
	Urinary (tubular) pole of a glomerulus
	Macula densa
	Extraglomular mesangial cells (Lacis cells) on vascular pole of a glomerulus
	Bowman capsular space
	Proximal tubule (proximal convoluted tubule)
	Distal convoluted tubule

Parts of the nephron in the renal cortex[sl]

1.	Arcuate artery
2.	Interlobular artery (cortical radiate artery)
3.	Afferent arteriole
4.	Distal convoluted tubule
5.	Connecting tubule
6.	Proximal tubule (proximal convoluted tubule)
7.	Efferent arteriole
8.	Straight medullary artery

Hematoxylin and Eosin (H&E)

H&E is the most common survey stain, which shows cell nuclei and cytoplasmatic components.

Structures	Colour
Nuclei	blue
Cytoplasm	pale red
Connective-tissue fibres - Collagen - Reticular - Elastic	red - pale rose to red
Muscle tissue	red
Erythrocytes	red-orange

Dyes:

Hematoxylin

(e.g. hemalaun, Weigerts or Heidenhains iron-hematoxylin)
Hematoxylin is a positively charged dye (at low pH values). It therefore colors negatively charged (basophilic) structures blue, like the DNA of nuclear chromatin, the RNA of rough endoplasmatic reticulum, and the acidic glycosaminoglycans of hyaline cartilage.
Hematoxylin used in the preparation of iron varnish is Weigerts or Heidenhains iron-hematoxylin and in the preparation of aluminum varnish is hemalaun.

Eosin

Eosin is a negatively charged dye used for counterstaining. It stains all remaining structures different shades of red (acidophilic).

Thiazinrot

Thiazinrot behaves similar as eosin.

Der distale Tubulus kehrt im Anschluss an die Henlesche Schleife wieder in die unmittelbare Nachbarschaft desjenigen Glomerulus zurück, von dem das Nephron ausgegangen ist. Er bildet dort am Gefäßpol die Macula densa. Diese ist daran zu erkennen, daß die Zellkerne des Epithels ungewöhnlich dicht stehen.

Magnification:

81x

Magnification:

160x

Magnification:

64x

Magnification:

128x

Capillaries and mesangium of glomerulus

Urinary (tubular) pole of a glomerulus

Extraglomular mesangial cells (Lacis cells) on vascular pole of a glomerulus

Bowman capsular space

Proximal tubule (proximal convoluted tubule)

Distal convoluted tubule

HistoNet2000 - Help

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3. Complementary information

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Drawing: schematic drawing of the specimen
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