Specimen Nr. 04D

Specimen:

Ileum (Human being)

Staining:

van Gieson

Magnification:

160x

Important structures :

1.Enterocytes of longitudinally-sliced villus
2.Goblet cell of longitudinally-sliced villus
3.Lamina propria of mucosa
4.Collagen fibrils of lamina propria
5.Fibrocytes and free connective tissue cells of lamina propria
6.Shrinkage artifact in lamina propria

Legende:

Enterocytes of longitudinally-sliced villus
Goblet cell of longitudinally-sliced villus
Lamina propria of mucosa
Collagen fibrils of lamina propria
Fibrocytes and free connective tissue cells of lamina propria
Shrinkage artifact in lamina propria

Villi and crypts (longitudinal slice)[bo]

1. Villi
2. Intestinal crypts (glands of Lieberkühn)
3. Simple columnar epithelium with microvilli (epithelial layer of mucosa)
4. Goblet cells
5. Lamina propria of mucosa
6. Lamina muscularis of mucosa
7. Lymphoid follicle
8. Central lymhatic vessel
9. Paneth's granular cells

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).
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Magnification:

28x

Magnification:

64x

Magnification:

160x

Magnification:

64x

Magnification:

160x

Magnifications
Enterocytes of longitudinally-sliced villus
Enterocytes of longitudinally-sliced villus
Goblet cell of longitudinally-sliced villus
Goblet cell of longitudinally-sliced villus
Goblet cell of longitudinally-sliced villus
Lamina propria of mucosa
Collagen fibrils of lamina propria
Fibrocytes and free connective tissue cells of lamina propria
Shrinkage artifact in lamina propria

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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