Specimen Nr. 04B

Specimen:

Ileum (Human being)

Staining:

van Gieson

Magnification:

28x

Important structures :

1.Epithelial layer of mucosa (villus)
2.Lamina propria of mucosa (villus)
3.Lamina muscularis of mucosa
4.Villus (oblique slice)
5.Shrinkage gap at apex of villus
6.Crypts (oblique slice)
7.Lymphoid follicle

Legende:

Epithelial layer of mucosa (villus)
Lamina propria of mucosa (villus)
Lamina muscularis of mucosa
Villus (oblique slice)
Shrinkage gap at apex of villus
Crypts (oblique slice)
Lymphoid follicle

Villi and crypts (longitudinal slice)[bo]

1. Villi
2. Intestinal crypts (glands of Lieberkühn)
3. Simple columnar epithelium with microvilli (epithelial layer of mucosa)
4. Goblet cells
5. Lamina propria of mucosa
6. Lamina muscularis of mucosa
7. Lymphoid follicle
8. Central lymhatic vessel
9. Paneth's granular cells

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).
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Magnification:

4x

Magnification:

28x

Magnification:

64x

Magnification:

160x

Magnification:

64x

Magnifications
Epithelial layer of mucosa (villus)
Epithelial layer of mucosa (villus)
Lamina propria of mucosa (villus)
Lamina propria of mucosa (villus)
Lamina muscularis of mucosa
Lamina muscularis of mucosa
Villus (oblique slice)
Shrinkage gap at apex of villus
Crypts (oblique slice)
Crypts (oblique slice)
Lymphoid follicle

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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