Specimen Nr. 01B

Specimen:

Femoral artery and vein (Cat)

Staining:

Azan

Magnification:

51x

Important structures :

1.	Lumen of artery
2.	Lumen of vein
3.	Membrana elastica interna
4.	Tunica media of artery
5.	Tunica adventitia of artery
6.	Tunica intima and tunica media of vein
7.	Tunica adventitia of vein

Es sind die Anschnitte einer Arterienwand und einer Venenwand zu sehen. Während die Arterie unter der Membrana elastica interna eine breite Tunica media mit vielen eng aneinanderliegenden ringförmig angeordneten glatten Muskelzellen (rot bei der Azanfärbung) aufweist, ist die Tunica media bei der Vene nur schmal ausgebildet. Die Tunica externa (Adventitia), die vor allem aus faserigem Bindegewebe mit kollagenen Fibrillen (blau bei der Azanfärbung) aufgebaut ist, überwiegt bei der Vene.

Legende:

	Lumen of artery
	Lumen of vein
	Membrana elastica interna
	Tunica media of artery
	Tunica adventitia of artery
	Tunica intima and tunica media of vein
	Tunica adventitia of vein

wall of an artery (muscular type)[wh]

1.	Intima
2.	Tunica media
3.	Adventitia

4.	Endothelium
5.	Membrana elastica interna

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Der Wandaufbau bei Arterien und Venen ist grundsätzlich gleich. Er besteht aus drei Schichten, die jedoch bei Vene und Arterie unterschiedlich ausgeprägt sind:

Tunica interna (Intima)
Tunica media (Media)
Tunica externa (Adventitia)

Magnification:

11x

Magnification:

51x

Magnification:

128x

Magnification:

128x

Membrana elastica interna

Tunica media of artery

Tunica adventitia of artery

Tunica intima and tunica media of vein

Tunica adventitia of vein

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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