Specimen Nr. 01A

Specimen:

Femoral artery and vein (Cat)

Staining:

Azan

Magnification:

11x

Important structures :

1.	Lumen of artery
2.	Lumen of vein
3.	Membrana elastica interna
4.	Tunica media of artery
5.	Tunica adventitia of artery
6.	Tunica intima and tunica media of vein
7.	Tunica adventitia of vein
8.	Peripheral nerve (cross section)
9.	Unilocular adipocytes

Die Übersichtsvergrößerung zeigt eine Arterie, Vene und periphere Nerven im Querschnitt, eingebettet in lockerem Bindegewebe mit vielen univakuolären Fettzellen. Die Wand der Vene ist zum Teil kollabiert, so dass ihr Lumen kleiner als das der Arterie erscheint. Es fällt der unterschiedliche Schichtenbau zwischen Vene und Arterie auf:
Die Tunica media ist bei der Arterie um ein vielfaches dicker als bei der Vene, während die Adventitia eher bei der Vene stärker erscheint. Die Intima ist bei dieser Vergrößerung nicht deutlich zu erkennen.

Legende:

	Lumen of artery
	Lumen of vein
	Membrana elastica interna
	Tunica media of artery
	Tunica adventitia of artery
	Tunica intima and tunica media of vein
	Tunica adventitia of vein
	Peripheral nerve (cross section)
	Unilocular adipocytes

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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