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Specimen Nr. 06B

Specimen:

Follicle of vermiform appendix (Dog)

Staining:

Azan

Magnification:

100x

Important structures :

1.Intestinal lumen
2.Epithelial layer of mucous membrane
3.Basement membrane
4.Germinal centre of a secondary lymphoid follicle
5.Area of M (microfold) cells
Die Abbildung zeigt die Dom-Region eines Sekundärfollikels und das darüberliegende Epithel bei starker Vergrößerung.

Legende:

Intestinal lumen
Epithelial layer of mucous membrane
Basement membrane
Germinal centre of a secondary lymphoid follicle
Area of M (microfold) cells

Peyer's patches of ileum[bo]

1. Villi
2. Intestinal crypts (glands of Lieberkühn)
3. Lymphoid nodule
4. Muscular layer

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic
blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G		 This dye is used for counterstaining.
Die den "Dom" bedeckende Lamina epithelialis der Tunica mucosa weist folgende Besonderheiten auf:
  1. Es gibt keine Krypten und Zotten.
  2. Es fehlen die Schleim-produzierenden Becherzellen.
  3. Die Epithelzellen produzieren nicht die sekretorische Komponente, so daß IgA nicht in das Darmlumen transportiert werden kann.
  4. Nur im Domepithel gibt es die M-Zellen. Sie besitzen an der basolateralen Oberfläche tiefe Einbuchtungen, in denen Lymphozyten zu finden sind, und transportieren Antigene vom Darmlumen zum lymphatischen Gewebe.
Außerdem hat die Basalmembran im Dom große Poren, durch die die Makrophagen ihre Zellfortsätze strecken.

Das Fehlen von Schleim und IgA erleichtert die Bindung von Antigenen aus dem Darmlumen am Dom-Epithel. Durch die Transportfunktion der M-Zellen können so große Moleküle trotz der Epithelbarriere in Kontakt mit Zellen des Immunsystems gelangen.

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Magnification:

50x

Magnification:

100x

Magnifications
Intestinal lumen
Epithelial layer of mucous membrane
Basement membrane
Germinal centre of a secondary lymphoid follicle
Area of M (microfold) cells

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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