Die stärkere Vergrößerung des Wurmfortsatzes (Appendix vermiformis) zeigt zum Darmlumen hin das einschichtige hochprismatische Epithel der Tunica mucosa, das die Krypten auskleidet. Als mehr isoprismatisches Dom-Epithel überdeckt es die Dom-Region der Lymphfollikel.

Legende:

	Intestinal lumen
	Epithelial layer of mucous membrane
	Germinal centre of a lymphoid follicle (secondary follicle)
	Area of M (microfold) cells
	Interfollicular area
	Crypts

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures	Colour
Nuclei	red
Cytoplasm	reddish
Connective-tissue fibres - Collagenic - Reticular - Elastic	blue blue unstained or reddish
Muscle tissue	red-orange

Dyes:

Azokarmin	Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue- orange G	This dye is used for counterstaining.

Die Lymphfollikel in der Lamina propria der Tunica mucosa und der Submucosa der Appendix vermiformis sind stets Sekundärfollikel mit einem aktiven Keimzentrum. In anderen peripheren lymphatischen Organen sieht man meist eine Mischung von aktiven sich entwickelnden und sich zurückbildenden Sekundärfollikeln, während in der Appendix vermiformis und den Peyerschen Platten alle Follikel aktiv sind. Bis zur Geburt gibt es im Verdauungstrakt nur Primärfollikel. Durch das spezialisierte Epithel, das die Primärfollikel zum Darmlumen hin bedeckt, wird der Kontakt mit Antigen im Bereich des Dom-Areals ermöglicht und so die Bildung von Sekundärfollikeln induziert.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 06A

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Azan (Heidenhain)

Dyes:

HistoNet2000 - Help