Specimen Nr. 02D

Specimen:

Small intestine (Human fetus)

Staining:

Azan

Magnification:

160x

Important structures :

1.Apical cell poles of enterocytes with brush border
2.Basal cell poles of enterocytes with vertically arranged cell nuclei
3.Goblet cell
4.Villous stroma with fibrocytes
Die hochprismatischen Enterozyten (Saumzellen) lassen am oberen Zellpol einen färberisch deutlich abgehobenen Saum erkennen. Dieser sog. Stäbchen- oder Bürstensaum entspricht den erst elektronenmikroskopisch identifizierbaren Mikrovilli. Die länglichen Zellkerne sind mehr basal gelegen. Vereinzelt sind Becherzellen, muköse Einzeldrüsen, in das Oberflächenepithel eingestreut.

Legende:

Apical cell poles of enterocytes with brush border
Basal cell poles of enterocytes with vertically arranged cell nuclei
Goblet cell
Villous stroma with fibrocytes

Cell junctions[bu]

1. Microvilli
2. Tight junction (occluding junction)
3. Adhesive belt (belt desmosome)
4. Gap junction
5. Desmosome

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic

blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G
This dye is used for counterstaining.

Mikrovilli sind fingerförmige Ausstülpungen der Zellmembran zur Oberflächenvergrößerung. Jeder Mikrovillus besteht aus einem zentralen Bündel aus Aktinfilamenten, die untereinander durch die Proteine Fimbrin und Fascin in ihrer Form gehalten werden. Zur lateralen Oberfläche wird das Aktinbündel durch "Minimyosin" und zum Zytoskelett nach basal durch Spektrin verbunden. Jeder Mikrovillus trägt am apikalen Ende eine amorphe Spitzenregion.

Die Mikrovilli überzieht eine Schicht aus Proteinen, Glykoproteinen und Zuckerresten, die Glykokalix. Sie verleiht den Zellen Antigeneigenschaften. Weiterhin spielt die Glykokalix bei Zellerkennungs- und Adhäsionsmechanismen eine Rolle.

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Magnification:

11x

Magnification:

64x

Magnification:

81x

Magnification:

160x

Magnifications
Apical cell poles of enterocytes with brush border
Apical cell poles of enterocytes with brush border
Basal cell poles of enterocytes with vertically arranged cell nuclei
Basal cell poles of enterocytes with vertically arranged cell nuclei
Goblet cell
Goblet cell
Villous stroma with fibrocytes
Villous stroma with fibrocytes
Villous stroma with fibrocytes

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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