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Specimen Nr. 02A

Specimen:

Small intestine (Human fetus)

Staining:

Azan

Magnification:

11x

Important structures :

1.Mucous membrane
2.Submucosa
3.Muscular layer
4.Serosa (peritoneum) and subserosa
5.Villi and crypts of the mucous membrane sliced obliquely
Der Querschnitt durch den Dünndarm eines menschlichen Fetus zeigt die fünf klassischen Schichten der Wand des Magen-Darm-Kanals: Tunica mucosa (Schleimhaut), Tela submucosa (Submucosa), Tunica muscularis (Muskelschicht), Tela subserosa (subseröse Bindegewebsschicht), Tunica serosa (Bauchfell). Die Zotten und Krypten der Tunica mucosa tragen an ihrer Oberfläche das einschichtige hochprismatische Epithel.

Legende:

Mucous membrane
Submucosa
Muscular layer
Serosa (peritoneum) and subserosa
Villi and crypts of the mucous membrane sliced obliquely

General structure of the digestive tract[bu]

1. Mesentery
2. Epithelial layer of mucosa
3. Lamina propria of mucosa
4. Lamina muscularis of mucosa
5. Mucous membrane
6. Submucosa
7. Circular layer
8. Longitudinal layer
9. Muscular layer
10. Serosa

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic
blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G		 This dye is used for counterstaining.
Der nahezu 5 m lange Dünndarm gewährleistet einen ausgiebigen Kontakt zwischen Nahrungsbestandteilen und Verdauungsenzymen. Die Nahrungsbestandteile kommen aus dem Magen, an den sich der Dünndarm anschließt. Die Verdauungsenzyme stammen aus dem Pankreas (Bauchspeicheldrüse), der in den ersten Abschnitt des Dünndarms, das Duodenum mündet. Die drei Abschnitte des Dünndarms sind: das Duodenum (Zwölffingerdarm), Jejunum (Leerdarm) und Ileum (Krummdarm). Auf den Dünndarm folgt als nächster Abschnitt des Verdauungstraktes der Dickdarm (Colon).
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Magnification:

11x

Magnification:

64x

Magnification:

81x

Magnification:

160x

Magnifications
Mucous membrane
Submucosa
Muscular layer
Serosa (peritoneum) and subserosa
Serosa (peritoneum) and subserosa
Villi and crypts of the mucous membrane sliced obliquely

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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