Specimen Nr. 07B

Specimen:

Dense connective tissue, Nunchal ligament (Cow)

Staining:

Hopa

Magnification:

128x

Important structures :

1.Parallel elastic fibres
2.Sharp-angled branches of elastic fibres
Auf dem Längsschnitt dieses straffen faserigen Bindegewebes erkennt man, wie die gelb gefärbten elastischen Fasern eng aneinander gedrängt über weite Strecken parallel verlaufen. Der typische Netzcharakter der elastischen Fasern ist an Stellen zu erkennen, wo sich diese spitzwinklig untereinander verbinden. Zwischen den elastischen Fasern liegt lockeres Bindegewebe (blau gefärbt) mit Fibrozyten.

Legende:

Parallel elastic fibres
Sharp-angled branches of elastic fibres

Dense fiberous connective tissue[bu]

1. Nuclei of fibrocytes
2. Cross section of fibre bundle
3. Longitudinal sliced fibre bundle

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Da bei den elastischen Bändern wie Ligamentum nuchae oder Ligamenta flava, aber auch in vielen aus straffem faserigen Bindegewebe bestehenden Gelenkbändern, die Zugbeanspruchung immer in der gleichen Richtung erfolgt, sind die Fasern der straffen Bindegewebe ganz überwiegend in einer bestimmten Richtung angeordnet. Der "Extremfall" des straffen faserigen Bindegewebes ist an der Sehne ausgebildet: Die kollagenen Fasern sind streng parallel angeordnet und zu Bündeln zusammengefaßt. Zwischen ihnen liegen die als Sehnenzellen bezeichneten Fibrozyten hintereinander aufgereiht.
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Magnification:

128x

Magnification:

128x

Magnifications
Parallel elastic fibres
Parallel elastic fibres
Sharp-angled branches of elastic fibres
Sharp-angled branches of elastic fibres
Sharp-angled branches of elastic fibres

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
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Help: Instructions for Use appear
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VM: provides virtual microscopy

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