Specimen Nr. 07A

Specimen:

Dense connective tissue, Nunchal ligament (Cow)

Staining:

Hopa

Magnification:

128x

Important structures :

1.Cross section of elastic fibres in loose connective tissue
2.Loose connective tissue between bundles of elastic fibres (cross section)
Dieser Querschnitt durch das Nackenband (Ligamentum nuchae) läßt die charakteristische Felderung dieses vorwiegend aus elastischen Fasern bestehenden Bandes erkennen. Die gelb gefärbten elastischen Fasern sind zu unterschiedlich großen Bündeln zusammengefaßt, unterteilt durch die hier blau gefärbten kollagenen und retikulären Fasern. Die spindelförmigen rot gefärbten Kerne der Fibrozyten sind zwischen den Bindegewebsfasern zu erkennen

Legende:

Cross section of elastic fibres in loose connective tissue
Loose connective tissue between bundles of elastic fibres (cross section)

Dense fiberous connective tissue[bu]

1. Nuclei of fibrocytes
2. Cross section of fibre bundle
3. Longitudinal sliced fibre bundle

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Elastische Bänder, aufgrund ihrer makroskopisch gelben Farbe auch "gelbe Bänder" genannt, gehören zum straffen Bindegewebe. Das klassische Beispiel für ein solches Band ist das Ligamentum nuchae der Huftiere. Beim Menschen kommen elastische Bänder als Ligamentum flava zwischen den Wirbelbögen vor. Auch das Stimmband, das Ligamentum vocale, besteht vorwiegend aus elastischen, annähernd parallel angeordneten Fasern.
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Magnification:

128x

Magnification:

128x

Magnifications
Cross section of elastic fibres in loose connective tissue
Cross section of elastic fibres in loose connective tissue
Cross section of elastic fibres in loose connective tissue
Loose connective tissue between bundles of elastic fibres (cross section)
Loose connective tissue between bundles of elastic fibres (cross section)
Loose connective tissue between bundles of elastic fibres (cross section)
Loose connective tissue between bundles of elastic fibres (cross section)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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