Specimen Nr. 03B

Specimen:

Adrenal gland (Suprarenal gland) (Human being)

Staining:

Hopa

Magnification:

16x

Important structures :

1.	Zone fasciculata of adrenal cortex
2.	Zona reticularis of adrenal cortex
3.	Medulla of adrenal (suprarenal) gland with chromaffin cells

Das Bild zeigt einen Ausschnitt mit Mark- und Rindengewebe. In beiden Anteilen sind Gruppen endokriner Zellen durch blaugefärbte retikuläre Fasern abgegrenzt.

Legende:

	Zone fasciculata of adrenal cortex
	Zona reticularis of adrenal cortex
	Medulla of adrenal (suprarenal) gland with chromaffin cells

Endocrine System

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Die Zellen des Nebennierenmarkes entsprechen entwicklungsgeschichtlich und aufgrund ihrer funktionellen Eigenschaften dem zweiten Neuron des Sympathicus. Sie empfangen cholinerge Synapsen und synthetisieren bzw. sezernieren Katecholamine, bilden allerdings selbst kein Axon und keine Synapsen. Gelegentlich findet man hier auch eine voll differenzierte sympathische Nervenzelle. Der Ausdruck "chromaffine Zellen" bezieht sich auf besondere Färbeeigenschaften der Nebennierenmarkszellen.

Magnification:

10x

Magnification:

16x

Magnification:

50x

Magnification:

50x

Zone fasciculata of adrenal cortex

Zona reticularis of adrenal cortex

Medulla of adrenal (suprarenal) gland with chromaffin cells

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!