Specimen Nr. 03A

Specimen:

Corpus luteum of pregnancy (Human being)

Staining:

Azan

Magnification:

4x

Important structures :

1.Original follicular cavity
2.Wall of corpus luteum with granulosa lutein cells and theca lutein cells
3.Follicular theca
4.Connective tissue infiltrating between the luteal cells
5.Invasive connective tissue bordering on the follicular cavity
6.Blood vessels

Legende:

Original follicular cavity
Wall of corpus luteum with granulosa lutein cells and theca lutein cells
Follicular theca
Connective tissue infiltrating between the luteal cells
Invasive connective tissue bordering on the follicular cavity
Blood vessels

Types of ovarian follicles

1. Primary ovarian follicle
2. Primary follicle
3. Secondary follicle
4. Large secondary ovarian follicle
5. Tertiary (Graffian) ovarian follicle
6. Ovulation
7. Corpora lutea
8. Atretic follicle
9. Medulla
10. Cortex
11. Blood vessels

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic

blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G
This dye is used for counterstaining.
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Boxes

Magnification:

4x

Magnification:

10x

Magnification:

32x

Magnification:

10x

Magnification:

26x

Magnifications
Original follicular cavity
Wall of corpus luteum with granulosa lutein cells and theca lutein cells
Follicular theca
Connective tissue infiltrating between the luteal cells
Connective tissue infiltrating between the luteal cells
Invasive connective tissue bordering on the follicular cavity
Invasive connective tissue bordering on the follicular cavity
Blood vessels

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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