Specimen Nr. 01C

Specimen:

Ovary (Cat)

Staining:

Azan

Magnification:

160x

Important structures :

1.Primary ovarian follicle
2.Stroma of ovarian cortex
Die Abbildung zeigt einen Primordialfollikel in der Ovarialrinde. Er besteht aus einer großen Oozyte, die von einer einzigen Schicht flacher Follikelepithelzellen umgeben ist.

Legende:

Primary ovarian follicle
Stroma of ovarian cortex

Primordial and secondary ovarian follicle[bo]

1. Primary ovarian follicle
2. Secondary follicle
3. Follicular epithelial cells
4. Cytoplasm of oocyte
5. Nucleus of oocyte
6. Follicular theca
7. Granulosa cells
8. Zona pelllucida
9. Oocyte

Azan (Heidenhain)

Azan dye is a multiple or trichromatic staining method. It differentiates extracellular connective-tissue fibres, tissues, and cell components.

Structures Colour
Nuclei red
Cytoplasm reddish
Connective-tissue fibres
- Collagenic
- Reticular
- Elastic

blue
blue
unstained or reddish
Muscle tissue red-orange

Dyes:

Azokarmin Azokarmin is a red dye used mainly to stain cell nuclei.
Aniline blue-
orange G
This dye is used for counterstaining.
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Magnification:

4x

Magnification:

41x

Magnification:

160x

Magnification:

256x

Magnification:

160x

Magnifications
Primary ovarian follicle
Stroma of ovarian cortex

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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