Specimen Nr. 06D

Specimen:

Skin of axilla (Human being)

Staining:

van Gieson

Magnification:

41x

Important structures :

1.Lumen of "empty" apocrine sweat gland tubule
2.Lumen of apocrine sweat gland tubule with columnar cells
3.Drops of cytoplasm containing secretions projecting into the lumen of an apocrine sweat gland
4.Secretion in the lumen of an intralobular duct
5.Myoepithelial cells
6.Eccrine sweat glands
7.Connective tissue (mostly elastic fibres)

Legende:

Lumen of "empty" apocrine sweat gland tubule
Lumen of apocrine sweat gland tubule with columnar cells
Drops of cytoplasm containing secretions projecting into the lumen of an apocrine sweat gland
Secretion in the lumen of an intralobular duct
Myoepithelial cells
Eccrine sweat glands
Connective tissue (mostly elastic fibres)

Apocrine secretion, sweat gland (Skin of axilla)[bu]

1. Constricting drop of secretion
2. Basement membrane
3. Myoepithelial cell
4. Glandular cell with double nucleus
5. Nucleus of myoepithelial cell
6. Capillary

Giesons trichromatic stain

This is the dye most commonly used to stain connective tissue.

Structures Colour
Nuclei black-brown
Cytoplasm yellow-brown
Connective-tissue fibres
- Collagen
- Reticular
- Elastic

red
-
yellow
Muscle tissue yellow
Erythrocytes yellow

Dyes:

Weigerts iron hematoxylin This dye is one of the hematoxylins, but stains much more intensively than hemalaun. It is used with van Gieson’s dye to stain cell nuclei.
Picro-fuchsin or Picro-thiacid mixture This dye solution simultaneously stains connective tissue red and muscle tissue yellow (simultaneous stain).

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Magnification:

13x

Magnification:

32x

Magnification:

41x

Magnification:

70x

Magnification:

102x

Magnifications
Lumen of "empty" apocrine sweat gland tubule
Lumen of "empty" apocrine sweat gland tubule
Lumen of apocrine sweat gland tubule with columnar cells
Lumen of apocrine sweat gland tubule with columnar cells
Drops of cytoplasm containing secretions projecting into the lumen of an apocrine sweat gland
Secretion in the lumen of an intralobular duct
Secretion in the lumen of an intralobular duct
Myoepithelial cells
Eccrine sweat glands
Connective tissue (mostly elastic fibres)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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