Specimen Nr. 02

Specimen:

Tongue (Cat)

Staining:

Hopa

Magnification:

11x

Important structures :

1.	Skeletal striated muscle (cross section)
2.	Skeletal striated muscle (sliced longitudinally)
3.	Skeletal striated muscle (sliced obliquely)
4.	Keratinized stratified squamous epithelium
5.	Filiform papilla
6.	Fungiform papilla
7.	Lamina propria of mucosa
8.	Adipose cells
9.	Serous glands (salivary glands)

Das unverhornte Plattenepithel der Schleimhaut des Zungenrückens zeigt hier zwei Arten von Zungenpapillen: die Papillae filiformes (fadenförmige Papillen) und die Papillae fungiformes (pilzförmige Papillen). Bei den Papillae filiformes handelt es sich um zipfelförmige Ausstülpungen des Epithels, deren Spitze verhornt ist. Sie sind zahlreicher als die Papillae fungiformes. Letztere sind breiter und pilzförmig, d.h. an der Basis schmäler. Sie weisen häufig Sekundärpapillen auf.

Legende:

	Skeletal striated muscle (cross section)
	Skeletal striated muscle (sliced longitudinally)
	Skeletal striated muscle (sliced obliquely)
	Keratinized stratified squamous epithelium
	Filiform papilla
	Fungiform papilla
	Lamina propria of mucosa
	Adipose cells
	Serous glands (salivary glands)

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!