Specimen Nr. 03

Specimen:

Spleen (Pig)

Staining:

Gomori silver stain

Magnification:

200x

Important structures :

1.	Red pulp
2.	Marginal zone of white pulp
3.	Central artery
4.	Periarteriolar lymphoid sheath (PALS)
5.	Primary lymphoid follicle
6.	Border between white pulp and marginal zone

Die Versilberung macht retikuläre Fasern (Kollagen Typ III) sichtbar und zeigt, daß alle Kompartimente der Milz charakteristische Anordnungen von retikulären Fasern aufweisen. Die meisten Fasern sind in der PALS zu sehen (parallel verlaufend), die wenigsten Fasern im Follikel. Durch viele retikuläre Fasern ist die weiße Pulpa von der Marginalzone abgegrenzt. Marginalzone und rote Pulpa zeigen ein wabenförmiges Muster, wobei das der Marginalzone feiner und das der roten Pulpa gröber organisiert ist.

Legende:

	Red pulp
	Marginal zone of white pulp
	Central artery
	Periarteriolar lymphoid sheath (PALS)
	Primary lymphoid follicle
	Border between white pulp and marginal zone

Localisation of organs of immune-lymphatic system[we]

1.	Pharyngeal tonsil
2.	Palatine tonsil
3.	Lymph node
4.	Lymphatic vessels

5.	Spleen
6.	Peyer's patches
7.	Thymus

Silver impregnation

In silver impregnation an ammoniacal silver-hydroxide solution is absorbed by tissue structures and precipitated by a reducing agent.

This results in a black stain of:

reticular (“argyrophilic”) fibres
neurofibrils
glial filaments

Die Organisation der retikulären Fasern spielt eine wichtige Rolle in der Aufrechterhaltung der verschiedenen Kompartimente der Milz. Wahrscheinlich bestimmen sie auch wesentlich die Route, die die Lymphozyten bei ihrer Wanderung (Migration) durch die Milz nehmen.

Marginal zone of white pulp

Periarteriolar lymphoid sheath (PALS)

Primary lymphoid follicle

Border between white pulp and marginal zone

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