Specimen Nr. 03A

Specimen:

Duct of epididymis (Sheep)

Staining:

Hopa

Magnification:

Important structures :

1.	Slices of epididymis
2.	Spermatocytes (clumpy)

Der stark verschlungen verlaufende Nebenhodengang ist hier in vielen Anschnitten zu sehen. Zwischen den Anschnitten sieht man glatte Muskelfasern und Bindegewebe. Im Lumen der Anschnitte liegen die verklumpten Samenzellen.

Legende:

	Slices of epididymis
	Spermatocytes (clumpy)

Epididymis

1.	Urinary bladder
2.	Ejaculatory duct
3.	Prostate
4.	Scrotum
5.	Testis
6.	Urethra
7.	Epididymis
8.	Seminal gland
9.	Ductus deferens

HOPA

Structures	Colour
Nuclei	brown
Cytoplasm	grey-blue
Connective-tissue fibres - Collagen - Reticular - Elastic	blue blue -
Myocytes	grey-blue to grey-green
Erythrocytes	yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der Nebenhodengang (Ductus epididymidis) hat eine Länge von vier bis sechs Meter. Er besteht aus dem Caput epididymidis (dicker, oberer Anteil im Anschluß an den Hoden), dem langgestreckten Corpus epididymidis, sowie der Cauda epididymidis, die dann in den Samenleiter (Ductus deferens) übergeht. Die Ringmuskelschicht aus glatter Muskulatur ist relativ dünn, erst am Übergang zum Samenleiter nimmt sie an Dicke zu, so daß deutliche Wandschichten entstehen.

Magnification:

64x

Magnification:

160x

Slices of epididymis

Spermatocytes (clumpy)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!