| 1. | Nucleolus |
| 2. | Nissl bodies |
| 3. | Base of a dendrite |
| 4. | Astrocyte |
| 5. | Capillaries (dilated) |
| 6. | Myelinated axon |
 | Nucleolus |
 | Nissl bodies |
 | Base of a dendrite |
 | Astrocyte |
 | Capillaries (dilated) |
 | Myelinated axon |
 |
Semi-thin sections are often stained by Richardson technique with a blue dye, Methylene blue Azure II.
Basophilic and osmiophilic structures stain blue, and metachromatic structures turn red-violet.
Semi-thin sections of tissue specimens are generally prepared for the light microscopic examination before ultra-thin sections are prepared for examination under the transmission electron microscope (TEM). Semi-thin sections are thinner than other sections commonly used for light microscopy.
The embedding agent for TEM examination is a plastic resin, which is harder than paraffin. Since plastic resin cannot be detached easily and without creating artifacts, in contrast to paraffin, there exist only few suitable staining techniques for tissue embedded in plastic resin. Richardson stain is one of them.
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1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!