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Specimen Nr. 01D

Specimen:

Smooth muscle tissue, Intestine (Axolotl)

Staining:

Hopa

Magnification:

128x

Important structures :

1.Longitudinal layer of muscular layer
2.Circular layer of the muscular layer
3.Connective tissue between layers of muscle tissue
4.Nucleus of smooth muscle cell
5.Lymphatic vessel
Die längs geschnittenen glatten Muskelzellen des Stratum circulare lassen deutlich die fein strukturierten stäbchenförmigen Zellkerne erkennen. Die Enden sind zum Teil geschlängelt oder korkenzieherartig gewunden. Das Zytoplasma der glatten Muskelzellen erscheint homogen und läßt keine Querstreifung erkennen. Jede der angeschnittenen polygonalen Zellen des Stratum longitudinale ist von feinem, blau gefärbten Bindegewebsfasern umgeben.

Legende:

Longitudinal layer of muscular layer
Circular layer of the muscular layer
Connective tissue between layers of muscle tissue
Nucleus of smooth muscle cell
Lymphatic vessel

Smooth muscle tissue[bu]

1. Cross section of smooth muscle cells
2. Nuclei of myocytes
3. Capillary lumen
4. Nucleus of endothelial cell
5. Nucleus of fibrocyte

HOPA

Structures Colour
Nuclei brown
Cytoplasm grey-blue
Connective-tissue fibres
- Collagen
- Reticular
- Elastic
blue
blue
-
Myocytes grey-blue to grey-green
Erythrocytes yellow-orange

Dyes:

HOPA = Hemalaun, Orange G, Phosphormolybdenic acid, Aniline blue

Cell nuclei are stained with hemalaun or Weigerts iron-hematoxylin and then counterstained with a dye mixture of orange-phosphormolybden with aniline blue.

Der glatten Muskelzelle fehlt im Vergleich zur Skelettmuskulatur die strenge Anordnung der kontraktilen Elemente, die aus Actin- und Myosinfilamenten bestehen. Diese verlaufen zusammen mit den Intermediärfilamenten kreuz und quer durch das Zytoplasma, bilden ein Netzwerk, dessen fokale Verdichtungen sich an der Zellperipherie verankern. Über die fokalen Verdichtungen werden bei der Kontraktion die Spannungen auf die die Muskelzellen umgebenden Laminae externae weitergegeben. So wirken viele glatte Muskelzellen als Funktionseinheit.
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Magnification:

11x

Magnification:

28x

Magnification:

77x

Magnification:

128x

Magnifications
Longitudinal layer of muscular layer
Circular layer of the muscular layer
Connective tissue between layers of muscle tissue
Nucleus of smooth muscle cell
Lymphatic vessel

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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