Abgebildet ist ein stark vergrößertes Osteon (Havers-System) eines Lamellenknochens. Im Zentrum des Osteons liegt der Havers-Kanal, umgeben von den regelmäßig konzentrisch angeordneten Knochenlamellen. Zwischen den Lamellen liegen die mandelkernähnlichen Lakunen der Osteozyten. Feine Verbindungskanälchen (Canaliculi) strahlen von den Lakunen aus und verbinden sich mit denen der Nachbarlakunen. Beim lebenden Knochen verlaufen in den Canaliculi die feinen Zytoplasmaausläufer der Osteozyten und verbinden sich über Nexus mit den Fortsätzen der Nachbarknochenzellen. Zur Umgebung ist das Osteon durch die sog. Kittfläche abgegrenzt, die nur selten von Knochenkanälchen durchsetzt ist.

Legende:

	Osteonic (Haversian) canal
	Part of osteon (Haversian system)
	Osteocyte lacuna
	Cement line (osteon border region)

Ground sections

Calcified bone is displayed in ground sections. Bone from which the calcium has not been removed is sawed into relatively thick slices. These are then ground thin and smooth, which destroys the organic components, like bone cells and blood vessels. The dye, e.g. basic fuchsin, infiltrates the resulting empty spaces which previously contained bone cells with their processes. This renders the architecture of the lamellar bone visible. The ground section is more or less intensively stained, depending on how well the dye has infiltrated the empty spaces.

Die über Nexus miteinander in Kontakt stehenden Zytoplasmaausläufer der Osteozyten liegen in kleinen Kanälchen, den Canaliculi. Diese Kanälchen sind Wege für die Zirkulation der Gewebsflüssigkeit und für die Diffusion von Stoffwechselprodukten zwischen den Lakunen und den Gefäßen des Havers-Kanals (Zentralkanal).

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Specimen Nr. 06D

Specimen:

Staining:

Magnification:

Important structures :

Legende:

Ground sections

HistoNet2000 - Help