Specimen Nr. 04D

Specimen:

Elastic cartilage, Epiglottis (Ape)

Staining:

Orcein-hemalaun

Magnification:

400x

Important structures :

1.Shrunken cartilage chondron
2.Interterritorial cartilage matrix
Zwischen den zu Chondronen zusammengelagerten Knorpelzellen erstreckt sich die extrazelluläre Matrix des elastischen Knorpels. Die elastischen Fasernetze sind durch die spezielle Elastika-Färbung dunkler angefärbt als die übrige Matrix. Die Chondrozyten sind zum Teil von der umgebenden Matrix gelöst, was auf Schrumpfungsvorgänge während der Präparation zurückzuführen ist.

Legende:

Shrunken cartilage chondron
Interterritorial cartilage matrix

Elastic cartilage[bu]

1. Chondrocyte
2. Pericellular matrix
3. Interterritorial matrix with elastic fibres

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres

Orcein

red-brown

Resorcin-fuchsin

blackish-blue to violet

Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

Durch die Einlagerung von elastischem Material in Form von Netzen oder elastischen Membranen erhält dieser Knorpel elastische Eigenschaften, die dem hyalinen Knorpel fehlen. Ebenso wie der hyaline Knorpel ist der elastische Knorpel von einem Perichondrium umgeben.
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Magnification:

4x

Magnification:

11x

Magnification:

44x

Magnification:

400x

Magnifications
Shrunken cartilage chondron
Interterritorial cartilage matrix
Interterritorial cartilage matrix
Interterritorial cartilage matrix

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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