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Specimen Nr. 04C

Specimen:

Elastic cartilage, Epiglottis (Ape)

Staining:

Orcein-hemalaun

Magnification:

44x

Important structures :

1.Elastic cartilage with chondrocytes
2.Perichondrium
3.Mucous end piece
4.Cartilage chondron
Bei stärkerer Vergrößerung heben sich die dunkler gefärbten elastischen Faserzüge von der übrigen, heller erscheinenden extrazellulären Matrix, ab. Die Knorpelzellen sind mehr oder weniger artifiziell verändert und liegen meist in Chondronen zusammen. Der elastische Knorpel ist durch das Perichondrium vom übrigen Bindegewebe abgegrenzt. In diesem sind die dicht gepackten, seromukösen Endstücke der Glandulae laryngeae zu erkennen.

Legende:

Elastic cartilage with chondrocytes
Perichondrium
Mucous end piece
Cartilage chondron

Elastic cartilage[bu]

1. Chondrocyte
2. Pericellular matrix
3. Interterritorial matrix with elastic fibres

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres
Orcein

red-brown
Resorcin-fuchsin

blackish-blue to violet
Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

Elastischer Knorpel enthält die gleichen Bausteine wie hyaliner Knorpel: Chondrozyten, Fibrillen, sowie Glykosaminoglykane (GAG) und Proteoglykane. Zusätzlich zu den Kollagen-Typ-II-Fibrillen kommt das ausgedehnte Netzwerk elastischer Fasern hinzu.
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Magnification:

4x

Magnification:

11x

Magnification:

44x

Magnification:

400x

Magnifications
Elastic cartilage with chondrocytes
Elastic cartilage with chondrocytes
Perichondrium
Mucous end piece
Mucous end piece
Cartilage chondron
Cartilage chondron
Cartilage chondron

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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