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Specimen Nr. 04B

Specimen:

Elastic cartilage, Epiglottis (Ape)

Staining:

Orcein-hemalaun

Magnification:

11x

Important structures :

1.Elastic cartilage
2.Seromucous laryngeal glands
3.Loose fibrous connective tissue with venous and lymphatic vessels
4.Artifact
5.Stratified squamous nonkeratinized epithelium (lingual side)
Bei diesem Ausschnitt der Epiglottis fällt der zentral liegende sich intensiver braunrot anfärbende elastische Knorpel auf. Die hellen Bezirke in ihm stellen die Knorpelzellen dar. Im angrenzenden lockeren faserigen Bindegewebe sind ausgedehnte Areale mit seromukösen Endstücken der Glandulae laryngeae zu sehen.

Legende:

Elastic cartilage
Seromucous laryngeal glands
Loose fibrous connective tissue with venous and lymphatic vessels
Artifact
Stratified squamous nonkeratinized epithelium (lingual side)

Elastic cartilage[bu]

1. Chondrocyte
2. Pericellular matrix
3. Interterritorial matrix with elastic fibres

Elastic Fibre Staining

A special staining technique used to identify elastic fibres. The table below shows the dyes which selectively stain elastic fibres.

Dyes

Colours of Elastic Fibres
Orcein

red-brown
Resorcin-fuchsin

blackish-blue to violet
Aldehydfuchsin

red to violet

Combinations with further dyes and staining techniques:

hemalaun

dye for staining cell nuclei
result: Cell nuclei turn intense blue.

nuclear fast red

used as a counterstain with Weigerts elastica dye
result: Cell nuclei turn red.

Elastischer Knorpel kommt in der Ohrmuschel, dem äußeren Gehörgang, in der Tulea auditiva, der Epiglottis und den kleinen Kehlkopfknorpeln vor.
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Magnification:

4x

Magnification:

11x

Magnification:

44x

Magnification:

400x

Magnifications
Elastic cartilage
Seromucous laryngeal glands
Loose fibrous connective tissue with venous and lymphatic vessels
Loose fibrous connective tissue with venous and lymphatic vessels
Artifact
Stratified squamous nonkeratinized epithelium (lingual side)
Stratified squamous nonkeratinized epithelium (lingual side)
Stratified squamous nonkeratinized epithelium (lingual side)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

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