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Specimen Nr. 10

Specimen:

Blood smear (Human being)

Staining:

Pappenheim

Magnification:

320x

Important structures :

1.Monocyte
2.Erythrocytes
Der typische Monozyt zeichnet sich durch einen nierenförmigen Zellkern aus, der exzentrisch in blau-grau angefärbtem, vakuolisiert erscheinendem Zytoplasma liegt. Das Zytoplasma enthält feine azurophile Granula, die Lysosomen entsprechen. Nucleoli, die zuweilen im Zellkern zu erkennen sind, sind hier nicht zu beobachten.

Legende:

Monocyte
Erythrocytes

Pappenheim stain

Pappenheim stain is a combination of May-Grünwald and Giemsa stains. They are the stains generally used for blood smears.

Structures Colour
Nuclei reddish-violet
Neutrophilic granules bluish-pink to brownish
Eosinophilic granules brick-red to brown-orange
Basophilic granules dark blue-violet
Lymphocytic cytoplasm light blue
Monocytic cytoplasm light blue
Erythrocytes pink
Thrombocytes blue with red-violet internal bodies

Dyes:

The May-Grünwald stain contains the dyes eosin and methylene blue.
The Giemsa stain contains the dyes eosin, methylene blue, methylene azure and methylene violet.

Monozyten sind die größten und vielgestaltigsten Zellen unter den Leukozyten. Sie gehören zum Mononukleären Phagozytensystem (MPS).
Monozyten wandern innerhalb von 2 bis 3 Tagen aus dem Blut ins umliegende Gewebe aus, wo sie sich in Abhängigkeit von ihrer Umgebung zu verschiedenen Typen von Makrophagen differenzieren. Sie gehören zum Schutzapparat des Körpers, sind gut amöboid beweglich und phagozytieren auch größere Strukturen, wie z.B. geschädigte oder abgestorbene Zellen und Zelltrümmer.
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Monocyte
Erythrocytes
Erythrocytes
Erythrocytes

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

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