Specimen Nr. 06

Specimen:

Eosinophilic granulocyte

Staining:

TEM

Magnification:

16400x

Important structures :

1.Segments of nucleus
2.Granules of eosinophilic granulocytes
Im organellenarmen Cytoplasma dieses eosinophilen Granulozyten sind zwei Kernsegmente des gelappten Zellkerns zu sehen. Die typischen, lichtmikroskopisch eosinophilen Granula erscheinen im Elektronenmikroskop als große ovale Gebilde, die zentral einen elektronendichten Kristallkörper enthalten, der von einer helleren Matrix umgeben ist

Legende:

Segments of nucleus
Granules of eosinophilic granulocytes

Transmission electron microscope (TEM)

The sections for examination under the transmission electron microscope are about 0.1µm thick. They are referred to as ultra-thin sections.

To obtain such ultra-thin sections, the tissues are embedded in plastic polymers like epon (instead of paraffin, which is used for examination under the light microscope) after fixation and dehydration. Ultra-thin sections are not stained with dyes, but contrasted with heavy-metal salts. The heavy-metal salts lead to a different electron scatter and thereby create a differentiated blackening of the photographic negative. A common method of creating contrast results with 5% uranyl acetate and lead citrate.

Die eosinophilen Granulozyten stellen nur 1-3% der weißen Blutkörperchen (Leukozyten). Ihre charakteristischen Granula sind Lysosomen, die eine Reihe von Enzymen enthalten. Ähnlich wie die anderen Granulozyten sind die Eosinophilen amöboid beweglich und zur Phagozytose fähig.
Sie beseitigen vor allem Antigen-Antikörper-Komplexe, die bei Immunreaktionen auftreten, während sie bei der unmittelbaren Abwehr von Bakterien und anderen Keimen wahrscheinlich nur eine geringe Rolle spielen.
Home
Tutor
Help
Exit
Boxes
Segments of nucleus
Segments of nucleus
Granules of eosinophilic granulocytes
Granules of eosinophilic granulocytes
Granules of eosinophilic granulocytes

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy

We hope you will enjoy working with HistoNet2000 and learn a lot from it!

Cose help