| 1. | Reticulocyte |
| 2. | Erythrocyte |
 | Reticulocyte |
 | Erythrocyte |
|
|
Vital staining refers to staining methods carried out on living animals or living cells. Intravital staining means staining is performed on a living object. Supravital staining is a method which is used on cells with limited vitality or tissue which has been removed from an animal.
The stain is usually applied by injection (intravenous, intraperitoneal, subcutaneous, or subdural) for live staining or by feeding (oral or enteral with a pharangeal tube). Supravital staining is carried out on organs or cells which have been kept alive and immersed in or perfused with a staining solution.
The available vital stains consist of:
Vital staining with Trypan blue
The dye is injected subcutaneously or intraperitoneally in vivo and stored by phagocytizing cells. It is also deposited where it is reabsorbed (like in the tubuli contorti of the kidneys).
Vital staining with India ink
Phagocytizing cells (like macrophages) can be selectively stained when they take up injected particles of India ink. Hollow spaces, like vessels and glandular ducts, can be demonstrated with this method.
Supravital staining with brilliant Cresyl blue
This dye is known for demonstrating the substantia granulofilamentosa of reticulocytes. Fresh blood smears are incubated with the basic vital dye, brilliant cresyl blue. After a few minutes a fine, granular or a filiform structure appears in some relatively large erythrocytes. These precipitations are primarily RNS artifacts.
| nuclear fast red | Depending on the dye solution, nuclear fast red can be used to stain the nucleus or plasma Result: Either nuclei or cell plasma turn red. |
1. Organization of the screen surface
Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)
2.Histologic specimen
Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.
3. Complementary information
Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated
4. General Program Functions
Home: returns you to the “start” page
Tutor: how to contact the HistoNet Team
Help: Instructions for Use appear
Exit: closes down the HistoNet program
Boxes: goes back to the other specimen of a topic
VM: provides virtual microscopy
We hope you will enjoy working with HistoNet2000 and learn a lot from it!