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Specimen Nr. 01A

Specimen:

Blood smear (Human being)

Staining:

Pappenheim

Magnification:

128x

Important structures :

1.Erythrocytes
2.Neutrophilic granulocyte
3.Basophilic granulocyte
4.Eosinophilic granulocyte
5.Lymphocyte
6.Thrombocytes
7.Monocyte
Dieses Blutausstrich-Präparat zeigt verschiedene kernhaltige weiße Zellen des peripheren Blutes inmitten blaßrot angefärbter kernloser Erythrozyten. Die Erythrozyten, die sich als bikonkave Scheiben mit blasser Zellmitte darstellen, besitzen keinen Zellkern und keine Zellorganellen. Beachten Sie die Relation der Größe von Erythrozyten (Durchmesser ~ 7,5 µm) im Verhältnis zu den kernhaltigen weißen Blutzellen.

Legende:

Erythrocytes
Neutrophilic granulocyte
Basophilic granulocyte
Eosinophilic granulocyte
Lymphocyte
Thrombocytes
Monocyte

Simplified scheme of origin of blood cells[gu]

1. Haematopoietic stem cell
2. Neutrophilic granulocyte
3. Eosinophilic granulocyte
4. Basophilic granulocyte
5. Erythrocyte
6. Megakaryocyte
7. Lymphocyte
8. Plasma cell
9. Monocyte
10. Macrophages

Pappenheim stain

Pappenheim stain is a combination of May-Grünwald and Giemsa stains. They are the stains generally used for blood smears.

Structures Colour
Nuclei reddish-violet
Neutrophilic granules bluish-pink to brownish
Eosinophilic granules brick-red to brown-orange
Basophilic granules dark blue-violet
Lymphocytic cytoplasm light blue
Monocytic cytoplasm light blue
Erythrocytes pink
Thrombocytes blue with red-violet internal bodies

Dyes:

The May-Grünwald stain contains the dyes eosin and methylene blue.
The Giemsa stain contains the dyes eosin, methylene blue, methylene azure and methylene violet.

Blut besteht aus einem flüssigen Anteil, dem Blutplasma und den darin suspendierten weißen und roten Blutzellen, den Leukozyten und Erythrozyten. Zu den geformten Blutbestandteilen gehören weiterhin die Blutplättchen, die Thrombozyten.

Als Hämatokrit bezeichnet man den Anteil der geformten Blutbestandteile am Blutvolumen. Er beträgt 41-46 %. Da die Gesamtheit der weißen Blutzellen (Granulozyten, Lymphozyten, Monozyten) nur ca. 1% der geformten Bestandteile ausmacht, wird er für gewöhnlich mit dem Anteil der Erythrozyten am Blutvolumen gleichgesetzt.
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Magnification:

128x

Magnification:

320x

Magnifications
Erythrocytes
Neutrophilic granulocyte
Neutrophilic granulocyte
Neutrophilic granulocyte
Basophilic granulocyte
Eosinophilic granulocyte
Lymphocyte
Thrombocytes
Monocyte

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

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