Specimen Nr. 04B

Specimen:

Pancreas (Human being)

Staining:

Silver

Magnification:

70x

Important structures :

1.	Reticular fibres surrounding exocrine pancreatic cells
2.	Reticular fibres (cross section)

An der Oberfläche der Drüsenzellen des exokrinen Pankreasanteils verlaufen retikuläre (argyrophile) Fasern, die durch die Silberimprägnation als feine schwarze Striche sichtbar sind. Die Pankreasdrüsenzellen sind auf Grund der hier angewandten Färbemethode nur unscharf zu erkennen.

Legende:

	Reticular fibres surrounding exocrine pancreatic cells
	Reticular fibres (cross section)

Reticular connective tissue surrounding adipocytes[bu]

1.	Reticular fibres
2.	Vacuole of adipocyte

Silver impregnation

In silver impregnation an ammoniacal silver-hydroxide solution is absorbed by tissue structures and precipitated by a reducing agent.

This results in a black stain of:

reticular (“argyrophilic”) fibres
neurofibrils
glial filaments

Retikuläre Fasern kommen in vielen Organen (z.B. Leber, Niere, Lunge, Drüsen) und Geweben (z.B. Endomysium, Endoneurium) als zartes Netz um die Zellen vor. Im Lymphknoten, der Milz und dem roten Knochenmark bilden retikuläre Fasern zusammen mit den sog. Retikulumzellen ein schwammartiges Stützgerüst, in das die immunologisch aktiven Zellen eingelagert sind. Erwähnenswert ist ihr Vorkommen in der Basalmembran.

Magnification:

70x

Reticular fibres surrounding exocrine pancreatic cells

Reticular fibres (cross section)

HistoNet2000 - Help

1. Organization of the screen surface

Right side: histologic specimen
Left side: information about the specimen (above) and general program functions (below)

2.Histologic specimen

Pull the mouse across the histologic specimen for training purposes. A small square with exclamation marks (dynamic labels) will appear where there is an important structure. You should then decide what structure this could be. To check your result, simply click the appropriate square, and the correct label will appear. The option “marked” allows you to see all labels for all structures simultaneously. These can be removed by clicking “unmarked”. This reactivates the dynamic labels.

3. Complementary information

Info: general information about the specimen, as well as a list of the dynamic labels
Drawing: schematic drawing of the specimen
Staining: information about the staining method for this specimen
Knowledge: short texts with basic histologic information, presently deactivated

4. General Program Functions

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Help: Instructions for Use appear
Exit: closes down the HistoNet program
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VM: provides virtual microscopy

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